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To make sound investment decisions, decision makers need accurate estimates of the uncertainties present in forecasts of reservoir performance. In this work I propose a method, the integrated mismatch method, that incorporates the misfit in the history match into the estimation of uncertainty in the prediction. I applied the integrated mismatch method, which overcomes some deficiencies of existing methods, to uncertainty estimation in two reservoir studies and compared results to estimations from existing methods. The integrated mismatch method tends to generate smaller ranges of uncertainty than many existing methods. When starting from nonoptimal reservoir models, in some cases the integrated mismatch method is able to bracket the true reserves value while other methods fail to bracket it. The results show that even starting from a ...
None of anticoccidial vaccines (Trivalent TAM™, monovalent Neca™ and imported pentavalent Paracox®-5) contain Eimeria brunetti in Japan, which has not been regarded as a cause of coccidiosis, because of its low prevalence. However, we have recently reported the evidence of a high nationwide prevalence of this species. In this report, we describe the characteristics of E. brunetti which have never been clearly defined in Japan. Mortality rates and other disease characteristics caused by our strain (Nb strain) were similar to those reported previously in other studies. Despite great reduction of body weight gains among groups infected with over 1 × 103 oocysts, the intestinal lesions in the infected chickens were rather mild compared to previous studies. Sulfa drugs and lasalocid were so effective that the E. brunetti infection was almos...
Toxoplasma gondii matrix antigen 1 (TgMAG1), known as the 65-kDa protein, which is abundantly expressed in both bradyzoites and tachyzoites, was evaluated as a candidate for the development of a diagnostic reagent for ovine toxoplasmosis. The TgMAG1 gene was expressed in Escherichia coli as a fusion protein with glutathione S-transferase (GST),and the recombinant TgMAG1 (rTgMAG1) was tested in an enzyme-linked immunosorbent assay (ELISA). The ELISA with rTgMAG1 showed a highly specific reaction with sera from mice experimentally infected with T. gondii but not with the closely related Neospora caninum. The antibodies to TgMAG1 were detectable from the acute to the chronic infectious stages in a mouse model. A total of 175 serum samples collected from sheep in 7 provinces of Mongolia were examined for the serodiagnosis of T. gondii infe...
The Taihang Mountain range of north-central China, the Southern region area of Fujian province, and the Chaoshan plain of Guangdong province are 3 major regions in China well known for their high incidence of esophageal cancer (EC). These areas also exhibit high incidences of gastric cardia cancer (GCC). The ancestors of the Chaoshanese, now the major inhabitants in the Chaoshan plain, were from north-central China. We hypothesized that EC and GCC patients in Chaoshan areas share a common ancestry with Taihang Mountain patients. We analyzed 16 East Asian-specific Y-chromosome biallelic markers (single nucleotide polymorphisms; Y-SNPs) and 6 Y-chromosome short tandem repeat (Y-STR) loci in 72 EC and 48 GCC patients from Chaoshan and 49 EC and 63 GCC patients from the Taihang Mountain range. We also compared data for 32 Chaoshan Hakka pe...
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