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Guanine-rich oligonucleotides often show a strong tendency to form supramolecular architecture, the so-called G-quadruplex structure. Because of the biological significance, it is now considered to be one of the most important conformations of DNA. Here, we describe the direct visualization and single-molecule analysis of the formation of a tetramolecular G-quadruplex in KCl solution. The conformational changes were carried out by incorporating two duplex DNAs, with G-G mismatch repeats in the middle, inside a DNA origami frame and monitoring the topology change of the strands. In the absence of KCl, incorporated duplexes had no interaction and laid parallel to each other. Addition of KCl induced the formation of a G-quadruplex structure by stably binding the duplexes to each other in the middle. Such a quadruplex formation allowed the...
The anisotropy of the electron velocity distribution function (EVDF) in plasmas can be deduced from the polarization of emissions induced by anisotropic electron-impact excitation. In this paper, we develop a compact thermal lithium atom beam source for spatially resolved measurements of the EVDF anisotropy in electron cyclotron resonance (ECR) plasmas. The beam system is designed such that the ejected beam has a slab shape, and the beam direction is variable. The divergence and flux of the beam are evaluated by experiments and calculations. The developed beam system is installed in an ECR plasma device with a cusp magnetic field, and the LiI 2s-2p emission (670.8 nm) is observed in low-pressure helium plasma. The two-dimensional distributions of the degree and direction of the polarization in the LiI emission are measured by a polariz...
会期・会場: 2011年7月3日(日)10:00-18:00 : 京都大学百周年時計台記念館 百周年記念ホール ; 後援: 読売新聞社、(財)京都大学教育研究振興財団
Based on the aerodynamic interaction between the heaving vortex-induced vibration and the torsional flutter, another explanation of the drastic change of the vibrational mode from the 5th symmetrical heaving mode to the 1st asymmetrical torsional mode is introduced. Some fundamental characteristics of the coupled flutter of a thin plate and a plate like structure, such as the flutter modes, the flutter branches, are explained by use of the “Step-by-Step flutter analysis”. The flutter properties obtained by the 3DOF multi-modes-flutter analysis are compared with the ones by the 2DOF 2modes- flutter analysis t. 3DOF multi-modes flutter analysis is recommended to use for more accurate verification of the flutter property as the lessons from the aerodynamic behavior of the full-scale elastic model of Akashi Kaikyo Bridge. The reduction of ...
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